The increased expression of NF-ΚB associated with carcinogenesis resulted in cell proliferation and stop apoptosis,in bladder carcinogenesis induced by dibutylamine / sodium nitrate mediated by e-coli infection. This study was designed to evaluate the potential protective effect of curcum in rats administered nitrosamine precursors; dibutylamine (DBA) and sodium nitrate (NaNO3); and infected with Escherichia coli (E. coli) and also to monitor changes in nuclear factor the Kappa B p65 (NF-ΚB p56) pathway and its downstream products, Bcl-2 and interleukin-6 (IL-6), in parallel with nitrosamine precursors, E. Coli and curcum treatment. Rats were divided into three groups (n = 25 each): Group I a normal control group, group II administered DBA/NaNO3 in drinking water and group III was administered DBA/NaNO3 in drinking water, infected with E. coli and receiving standard diet containing 1% curcum powder. and group IV received DBA/NaNO3 +E.coli+ curcum. Dibutylamine was given as 1000 ppm, NaNO3 was given as 2000 ppm in drinking water, infected by 0.1 ml saline containing suspension of E. coli in the bladder (approximately 2 X 10⁶ organisms) and received standard diet containing 1% curcum powder mixed in the diet. Histopathological examination and immunohistochemical studies reflected that the curcum treated group featured a lower incidence of urinary bladder lesions, and lower levels of NF-ΚB, Bcl-2 and IL-6 and Ki67 protein than the group receiving nitrosamine precursor and infected with E. coli. These findings suggested that curcum may have a protective role during the process of bladder carcinogenesis by inhibiting the NF-ΚB pathway and its downstream products.

Keyword:Bladder carcinogenesis; Curcum; E.coli; DBA; NF-ΚB, Ki67; Immunohistochemistry; Caspase-3.

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