Acinetobacter baumannii, as important opportunistic pathogens, have been found to be responsible for an increasing number of Multiple-drug resistant(MDR) nosocomial infections in worldwide. In the present study, various isolates of MDR, obtained from different regions of intensive care unite (ICU) from Riyadh hospitals , KSA were analyzed for antimicrobial susceptibilities, plasmid profile and carried out the molecular typing by multiplex PCR to elucidate and determine the resistance gene associated with plasmid and chromosomal DNA of A . baumannii. Seventy six strains of multidrug-resistance A. baumannii were isolated. The bacterial DNA of all isolates were subjected to the multiplex PCR to detect genes encoding oxacillinases and metallo-b-lactamases in carbapenem-resistant Acinetobacter spp. Multi-drug resistant isolates of A. baumannii obtained from different regions of intensive care units have been included in this study. These isolates showed resistance hundred percent to Ticarcillin-clavulanate, Meropenem Aztreonam, Ceftazidime, Cefoprazone, Ceftizoxime, Ceftazidime and Carbenicillin antibiotics. Some strains were also found resistant to Amikacin, Tobramycin Gentamicin with 41% ,33% ,23% . Other isolates were sensitive to some antibiotics with different percent. The plasmids from bacterial strains were found to be higher than 194 kb, harboring antibiotic resistance genes. A multiplex PCR was used to identify antibiotic resistance genes encoding the MDR phenotypes in clinical isolates of A. baumannii. A homogenous pattern of multiplex PCR product revealed that MDR isolates of A. baumannii harbored the same resistance genes. This study will help taking effective measures for controlling disease in the Riyadh region, and the data could be used for future medical reference.

Keyword:Acinetobacter baumannii, Carbapenem resistance, Oxa-51-like-F, plasmid. metallo-b-lactamases

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